Uses And Principle Of Operation Of Biosafety Cabinet

Product protection to avoid contamination of the work experiment or process.

Uses and principle of operation of biosafety cabinet.

Biological safety cabinet a piece of equipment designed to protect the operator the laboratory environment and work materials from exposure to infectious aerosols and splashes that may be generated when manipulating substances containing infectious agents such as viruses bacteria and primary tissue cultures. Primary containment which is the protection of personnel and the immediate laboratory environment from exposure to infectious agents is provided. The video describes the biosafety cabinet its principles operation uses maintenance calibration and safety methods. Follow these guidelines for working safely in a biological safety cabinet bsc.

With proper techniques activities within the work area can be performed safely minimizing the chance of cross contamination. This chapter discusses the purpose principle of operation specifications and applications of biosafety cabinet bsc. The purpose of containment is to reduce or eliminate the exposure to potentially hazardous agents of laboratory workers other persons and the outside environment. Improper use of a biological safety cabinet can result in contaminated cultures and expose workers to infectious organisms.

Personnel protection from harmful agents inside the cabinet. Use of biological safety cabinets bsc conduct all procedures involving the manipulation of potentially infectious materials including aerosol producing activities when using aerosol producing equipment or when using high concentrations large volumes of organisms inside a biological safety cabinet. Improved principles ofbiological safety cabinet design true laminar airflow unidirectional airflow moving along parallel flow lines at a constant velocity minimizes air turbulence within the work zone. A biosafety cabinet bsc also called a biological safety cabinet or microbiological safety cabinet is an enclosed ventilated laboratory workspace for safely working with materials contaminated with or potentially contaminated with pathogens requiring a defined biosafety level.

A bsc serves as a means to protect the laboratory worker and the surrounding e. In varying degrees a laminar flow biological safety cabinet is designed to provide three basic types of protection. The air mixes with the recirculating air stream and passes through a hepa filter downward toward the work surface creating a contamination free zone. The terms biological safety cabinet and biosafety cabinet have been widely used to describe a variety of containment devices equipped with hepa filter s designed to provide personnel or both personnel and product protection from biohazardous materials.